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Sino Biological
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MedChemExpress
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Databank Inc
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OriGene
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Human Protein Atlas
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Human AGO2 Protein Lysate 20ug from Innovative Research is provided as a Lyophilized powder. This is a Recombinant Protein Lysate produced in HEK293T cells. This protein lysate can be reconsituted using SDS Sample Buffer. Once
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The Recombinant Human Ago2 eIF2C2 Protein has been validated for the following applications Western Blot ELISA Protein Array Immunoaffinity Purification
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Recombinant Human Ago2/eIF2C2 GST (N-Term) Protein
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Image Search Results
Journal: Frontiers in Veterinary Science
Article Title: Analyzing the interactions of mRNAs, miRNAs and lncRNAs to predict ceRNA networks in bovine cystic follicular granulosa cells
doi: 10.3389/fvets.2022.1028867
Figure Lengend Snippet: Analysis of miR-664b sponge HSD17B7 3'-UTR and lncRNA NONBTAT027373.1. (A) : The networks of lncRNA-miRNA- HSD17B7 (B) : The expression of DE lncRNAs in lncRNA-miRNA- HSD17B7 networks. (C) : The expression of HSD17B7 mRNA. (D) : The expression of DE miRNAs in lncRNA-miRNA- HSD17B7 networks. (E) : Interaction between lncRNA NONBTAT027373.1 and miR-664b through Ago2 protein investigated using the RIP assay. lncRNA NONBTAT027373.1 and miR-664b expression quantified by RT-qPCR. (F) : Schematic view of the miR-664b seed region, the motif of HSD17B7 3'-UTR and lncRNA NONBTAT027373.1. (G,H) : Construction of WT and MUT plasmid vectors of HSD17B7 3'-UTR and lncRNA NONBTAT027373.1. (I) : Dual-luciferase reporter gene analysis of the regulatory relationship between HSD17B7 3'-UTR and miR-664b. (J) : Dual-luciferase reporter gene analysis of the regulatory relationship between lncRNA NONBTAT027373.1 and miR-664b. (K) : Transfection efficiency of miR-664b after overexpression and knockdown of miR-664b. (L) : miR-664b regulates the expression of HSD17B7 mRNA. (M) : RT-qPCR analysis of the effect on silence of lncRNA NONBTAT027373.1 expression by siRNA. (N) : Relative expression of HSD17B7 mRNA in groups. NS, Non-significant. Values represent means ± SEM for three individuals. * P < 0.05, ** P < 0.01. Yellow nodes represent HSD17B7 ; red nodes represent miRNAs and blue nodes represent lncRNAs in lncRNA-miRNA- HSD17B7 networks. WT, Wild Type; MUT, Mutant; Mimics, Overexpression of miR-664b; Inhibitor, Knockdown of miR-664b; NC, Negative control.
Article Snippet: Incubated A/G protein beads (
Techniques: Expressing, Quantitative RT-PCR, Plasmid Preparation, Luciferase, Transfection, Over Expression, Mutagenesis, Negative Control